ACTIVATED PENETRATIN 1
The Active Penetratin™ 1 Peptide Transfection reagent features a patented 16 amino acid peptide corresponding to the third helix of the homeodomain of the Antennapedia protein (pAntp) from Drosophila melanogaster.
Activated Penetratin™ 1 Peptide is a novel tool for peptide and siRNA delivery. It has been successfully used to internalize covalently attached peptides, oligonucleotides, and siRNA. It is sold ready for coupling to peptides or oligonucleotides containing thiol (-SH) functions.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
|Alternate Names||Transfection Reagent|
|Application Notes||Activated Penetratin™ 1 Peptide is a novel tool for peptide and siRNA delivery. It has been successfully used to internalize covalently attached peptides, oligonucleotides, and siRNA. It is sold ready for coupling to peptides or oligonucleotides containing thiol (-SH) functions.|
|Cells Tested||Adherent and suspension cell lines|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Lyophilized Solid|
|Product Overview||Penetratin™ 1 is a patented 16-amino acid peptide corresponding to the third helix of the homeodomain of antennapedeia protein. This peptide is able to translocate across biological membranes by an energy-independent mechanism (1-3) and has been used successfully to internalize covalently attached peptides (4), oligonucleotides (5) and siRNA (6). Activated Penetratin™ 1 is sold ready for coupling to peptides or oligos containing thiol (-SH) functions. Biotinylated Activated Penetratin™ 1 possesses a biotin group at the C-terminal end allowing the detection of the internalized molecule with an appropriate avidin or streptavidin reagent.|
|References||1. Damante G. et al., (1996) EMBO J. 15, 4992-500||2. Dorn A. et al. (1994) Pharmac. Ther. 61,155-183||3. Prochiantz A. (1996) Curr. Opin. Neurobiol. 6, 629-634||4. Derossi D. et al., (1996) J Biol. Chem. 271,18188-18193||5. Brian R et al., (2004) J. Exp. Med 4,547-557||6. Davidson T.J. et al., (2004) J. of Neuroscience. 24 (45), 10040-10046|
|Research Areas||Cell Viability & Cytotoxicity||Cell Cycle||Cell Metabolism|
|Storage and Handling||Stable for two years when frozen (< -15 °C).|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|