}{ TAQ-& LOAD PCR MASTERMIX

}{ TAQ-& LOAD PCR MASTERMIX

Synonyms Mastermix PCR Kit with loading dye

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SKU 11EPTAL100-CF
Price:
$168.90
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Description

Product Description

Taq-&LOAD™ PCR mastermix allows direct loading after the PCR on the agarose gel without the need of loading dye

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Routine PCR

Specifications
SKU 11EPTAL100-CF
Alternate Names Mastermix PCR Kit with loading dye
Brand Taq-&LOAD™
Certifications Free of RNases and endo- and exonucleases
Components Recombinant Taq DNA Polymerase |dNTPs |MgCl2, |incubation and loading buffer (includes a compound increasing the density of the sample and a red/purple dye)
Format Kit
Grade Molecular Biology Grade
Applications Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Routine PCR
Kit Compatibility Any DNA Isolation or Purification Kit
Kit or Assay Type PCR Kits
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Enzyme and reagents
Presentation Kit
Product Families Description All MP Biomedicals thermostable DNA polymerases are available in ready-to-use &GO™ PCR mastermix formats. &GO™ PCR Mastermixes contain all the components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the risk of pipetting and reagent handling errors. Each &GO™ mix is provided 5X concentrated and can be used to perform 20 μL or 50 μL reactions. To prepare the reaction, simply add 10 μL of &GO™ PCR Mastermix 5XC to template and primers diluted to 40 μL. For smaller reactions use 4 μL of mastermix in a 20 μL reaction.
Product Overview Taq-&LOAD™ is a 5X concentrated PCR mastermix containing MP Biomedicals recombinant Taq DNA Polymerase, dNTPs, MgCl2, incubation and loading buffer (includes a compound increasing the density of the sample and a red/purple dye). This mastermix allows direct loading after the PCR on the agarose gel without the need of loading dye. The included red purple dye migrates at 400bp on a 1,2% agarose gel.
Protein or Enzyme Type Molecular Biology Enzymes
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics
Storage and Handling Store at -20 °C.
Unit Definition One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.