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ISIS CORE KIT 10 MM

ISIS CORE KIT 10 MM

Synonyms High Fidelity Polymerase |Proofreading DNA Polymerase

EC Number: 232-741-2

Packaging size: 100 U

SKU 11EPSISK100
Price:
$167.55
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Description

Product Description

ISIS CORE KIT (with 10 mM dNTPs )

Application Notes

Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR

Specifications
SKU 11EPSISK100
Application Notes Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
Brand Isis™
Certifications Free of RNases and endo- and exonucleases
Components 100 U Isis™ DNA Pol at 1 U/µL |dNTPs at 10 mM each |Isis™ buffer 10xC (1mL) with MgSO4 |1 mL MgSO4 at 30 mM |dNTPs 10 mM
Concentration 1 U/µl
EC Number 232-741-2
Format Kit
Grade Molecular Biology Grade
Applications Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR
Kit Compatibility Any DNA Isolation or Purification Kit
Kit or Assay Type PCR Kits
Molecule Enriched DNA
Pack Size 100 U
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Enzyme and reagents
Presentation Kit
Product Overview Isis™ DNA Polymerase is the most thermostable proofreading polymerase available for high fidelity PCR. Isis CORE Kit 10 contains all the reagents (presented as separate items) required for PCR including: Isis DNA polymerase, high purity dNTPs Mix (>99%), optimized incubation buffers with and without MgCl2 and a separate MgCl2.
Protein or Enzyme Type Molecular Biology Enzymes
References (1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics
Storage and Handling Store at −20 °C.
Unit Definition One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.