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SUREPRIME CORE KIT 10

SUREPRIME CORE KIT 10

Synonyms Hot Start PCR Kit

Packaging size: 250 U

SKU 11EPHSK105
Price:
$960.20
In Stock
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Description

Product Description

SurePRIME CORE KIT 10 (with dNTPs at 10 mM each)

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Multiplex PCR |RAPD |Random Amplified Polymorphic DNA

Specifications
SKU 11EPHSK105
Brand SurePRIME™
Certifications Free of RNases and endo- and exonucleases
Components SurePRIME™ DNA polymerase |high purity dNTPs Mix (>99%) |optimized incubation buffers
Concentration 5 U/μL
Format Kit
Grade Molecular Biology Grade
Applications Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Multiplex PCR |RAPD |Random Amplified Polymorphic DNA
Kit Compatibility Any DNA Isolation or Purification Kit
Kit or Assay Type PCR Kits
Molecule Enriched DNA
Pack Size 250 U
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Enzyme and reagents
Presentation Kit
Product Families Description SurePRIME™ DNA Polymerase is a thermostable polymerase that is suitable for all PCR applications requiring “hot start” conditions. The enzyme is a highly purified form of recombinant Taq polymerase that has been chemically modified by the addition of heat-labile blocking groups to specific amino acid residues. |Heat activation for Specificity SurePRIME™ DNA Polymerase is heat-activated after a preincubation step of 15 min at 95°C after which it is functionally equivalent to classical Taq DNA Polymerase. Linear activation is also possible (5 to 7 min at 95°C as initial activation and additional 1 min at 95°C for the fi rst 7-8 PCR cycles). In its inactive state, SurePRIME™ DNA Polymerase is incapable of extending primer-dimers or mis-annealed primer-template species, therefore PCR performed after activation is highly specific. In laboratory tests which deliberately accentuate problems commonly encountered in PCR (i.e. nonspecific hybridization, primer-dimer formation) SurePRIME™ DNA Polymerase easily outperforms Taq DNA polymerase.
Product Overview SurePRIME™ CORE Kits contain all the reagents (presented as separate items) required for Hot Start PCR : MP Biomedicals SurePRIME™ DNA polymerase, high purity dNTPs Mix (>99%) and optimized incubation buffers.
Protein or Enzyme Type Molecular Biology Enzymes
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics
Storage and Handling Store at -20C.
Unit Definition One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.