Dino

FastDNA-96 Fungal/Bacterial DN

FastDNA-96 Fungal/Bacterial DN

Packaging size: 2 x 96 Preps

SKU 119696300
Price:
$1,032.60
In Stock
Request a quote
Description

Product Description

The FastDNA™-96 Fungal and Bacterial DNA Kit is designed for the simple and rapid isolation of, PCR-quality DNA from tough-to-lyse fungi and bacteria in approximately 40 minutes. Gram(+/-) bacteria, fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S.pombe, & mycelium, as well as spores, nematodes, pollen, and even mammalian cells are easily lysed in approximately 60 seconds with the FastPrep-96™ Instrument.

Application Notes

FastDNA™-96 Fungal/Bacterial DNA Kit is used for rapid isolation of PCR-quality DNA from tough-to-lyse fungi and bacteria in approximately 40 minutes.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used for Isolation of PCR-Ready Genomic DNA from Fungi and Bacteria Samples

Specifications
SKU 119696300
FastPrep Instruments Models 2 x 96 Well Adapter
Application Notes FastDNA™-96 Fungal/Bacterial DNA Kit is used for rapid isolation of PCR-quality DNA from tough-to-lyse fungi and bacteria in approximately 40 minutes.
Components FastDNA-96™ Lysing Matrix Rack (0.5 mm Beads) 2 x 96-well rack, Lysis Buffer 2 x 40 mL, Fungal/Bacterial DNA Binding Solution 150 mL, Binding Plate Pre-Wash Buffer 50 mL, Fungal/Bacterial DNA Wash Buffer 100 mL, Elution Solution 2 x 10 mL, Deep-Well Plate 2 each, MP-96 Binding Plate 2 each, Collection Plate 2 each, Elution Plate 2 each, Foil Plate Cover 4 each.
Applications Used for Isolation of PCR-Ready Genomic DNA from Fungi and Bacteria Samples
Material of Construction Matrix Y, 0.5 mm diameter Yttria-Stabilized Zirconium Oxide
Materials Required Not Supplied FastPrep-96™ Instrument or alternative high speed disruptor, Centrifuge with a swing-bucket style rotor that can spin up to 3,500 – 5,000 rpm, Swing-bucket centrifuge microplate adaptors for 96-well plates (pair), Plate shaker, Sterile water.
Molecule Enriched DNA
Pack Size 2 x 96 Preps
pH Reagent: Lysis Buffer Lysis Buffer- pH: 8.5 - 9.5, Reagent: Fungal/Bacterial DNA Binding Solution Fungal/Bacterial DNA Binding Solution- pH Range: 8.0, Reagent: Binding Plate Pre-Wash Buffer Binding Plate Pre-Wash Buffer-pH Range: 7.0 - 8.0, Reagent: Fungal/Bacterial DNA Wash Buffer Fungal/Bacterial DNA Wash Buffer - pH Range: 7.5 - 8.0, Reagent: Elution Solution Elution Solution- pH Range: 7.0 - 8.0.
Product Overview Up to 10-20 mg of wet weight fungal or bacterial cells (approximately 2x108 bacterial cells or 2x107 yeast cells), or up to 40 mg soft animal tissue or Nematoda (2x106 mammalian cells) are placed into each 1.2 ml tube of a FastDNA-96™ Lysing Matrix Rack ( 96-deep well plate) containing 0.5 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse all organisms including historically difficult sources such as eubacterial spores and endospores, gram (+/-) bacteria, yeast, algae, molds and fungi, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis and PCR (A260/A280 ratios = 1.8). Yield is typically 5 µg of total DNA eluted in 50-100 µL of elution solution (25 mL minimum). This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
Sample Type Bacteria (Gram +/-); Yeast; Fungi; Spores; Algae
Storage and Handling All FastDNA-96® Fungal & Bacterial DNA Kits are shipped ambiently. All FastDNA-96® Fungal & Bacterial DNA Kit reagents are stable at room temperature (15-30 °C).
Tube Compatibility 1.2 mL in 96 Deep Rack
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.