FastDNA-96 Soil Microbe DNA Kit
The FastDNA-96™ Soil Microbe DNA Kit quickly and efficiently isolates humic-free, PCR-ready genomic DNA from soil samples in approximately 50 minutes. Gram(+/-) bacteria, fungi, plant and animal tissues, algae, spores and many other members of a soil population including clay, sandy, silty, peaty, chalky, and loamy soils, are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument.
The FastDNA-96™ Soil Microbe DNA Kit quickly and efficiently isolates humic-free, PCR-ready genomic DNA from soil samples in approximately 50 minutes.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Used for Isolation of PCR-Ready Genomic DNA from Soil Samples
|FastPrep Instruments Models||2 x 96 Well Adapter|
|Application Notes||The FastDNA-96™ Soil Microbe DNA Kit quickly and efficiently isolates humic-free, PCR-ready genomic DNA from soil samples in approximately 50 minutes.|
|Components||FastDNA-96™ Lysing Matrix Rack (0.5 mm Beads) 2 x 96-well rack, Lysis Buffer 2 x 40 mL, Soil DNA Binding Solution 150 mL, Binding Plate Pre-Wash Buffer 50 mL, Soil DNA Wash Buffer 100 mL, Elution Solution 2 x 10 mL, Inhibitor Plate Prep Solution 30 mL, Deep-Well Plate 2 each, MP-96 Binding Plate 2 each, MP-96 Inhibitor Removal Plate 2 each, Collection Plate 2 each, Elution Plate 6 each, Foil Plate Cover 4 each.|
|Applications||Used for Isolation of PCR-Ready Genomic DNA from Soil Samples|
|Material of Construction||Matrix Y, 0.5 mm diameter Yttria-Stabilized Zirconium Oxide|
|Materials Required Not Supplied||FastPrep-96™ Instrument or alternative high speed disruptor, Centrifuge with a swing-bucket style rotor that can spin up to 3,500 – 5,000 rpm, Swing-bucket centrifuge microplate adaptors for 96-well plates (pair), Plate shaker, Sterile water.|
|pH||Reagent: Lysis Buffer Lysis Buffer-pH: 8.0, Reagent: Soil DNA Binding Solution Soil DNA Binding Solution- pH Range: 8.5 - 9.5, Reagent: Binding Plate Pre-Wash Buffer Binding Plate Pre-Wash Buffer-pH Range: 7.0 - 8.0, Reagent: Soil DNA Wash Buffer Soil DNA Wash Buffer- pH Range: 7.5 - 8.0, Reagent: Elution Solution Elution Solution- pH Range: 7.0 - 8.0, Reagent: Inhibitor Plate Prep Solution Inhibitor Plate Prep Solution- pH Range: 6.0 - 7.0.|
|Product Overview||Up to 130 mg of soil are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack (96-deep well plate) containing 0.5 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram (+/-) bacteria, yeast, algae, nematodes, and fungi, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes humic acids, polyphenols that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis and PCR (A260/A280 ratios = 1.8). Yield is typically 5 µg of total DNA eluted in 50-100 µL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.|
|Research Areas||OTHER - Drug Discovery; OTHER - Soil & Environmental Science|
|Sample Type||Soil; Feces; Environmental Water; Wastewater; Sludge|
|Storage and Handling||All FastDNA-96® Soil Microbe DNA Kits are shipped ambiently. All FastDNA-96® Soil Microbe DNA Kit reagents are stable at room temperature (15-30 °C).|
|Tube Compatibility||1.2 mL in 96 Deep Rack|