Double dropout formulation (without Leucine and Tryptophan) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Application Notes||The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Clarity NTU||Clear to Slightly Hazy|
|Color APHA||Colorless to Faint Yellow|
|Media Type||Yeast Media|
|Formulation Details||Contents per Liter: Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 20 mg |L-Isoleucine 50 mg |L-Leucine 0 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 0 mg |Uracil 20 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 640.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out.|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM or CSM-Dropout formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.79g of CSM or the appropriate weight of CSM-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.|
|Product Families Description||Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. MP Biomedicals offers many amino acid dropout media through combinations of our Complete Supplement Mixture (CSM), several carbon sources, and variations of YNB. If you need amino acid ratios different from those found in our CSM mixtures, we also offer classic amino acid formulations Hollenberg Supplement Mixture (HSM), Brent Supplement Mixture (BSM) and Synthetic Complete/Hopkins Mixture (SC). Synthetic defined media can be ordered in a number of ways: - Order a complete SD or SDA - Order a dropout Base (DOB) or Dropout Base with Agar (DOBA) plus the appropriate amino acid supplement mixture (CSM) - Order a Yeast Nitrogen Base (YNB), a carbon source, the appropriate amino acid supplement mixture (CSM), and agar if desired. - Order all raw materials separately Synthetic Defined Yeast Media: Formulas To Grow By! Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. Use the following table to navigate through the world of yeast media choices! | SDA = SD recipe + 17 g/L of Agar; DOBA = DOB recipe + 17 g/L of Agar |Use Yeast Nitrogen Base (YNB) Formulations to make your own custom synthetic defined yeast media. Start with the YNB of your choice, add a carbon source, the appropriate amino acids, and agar if necessary. |Dropout Base (DOB) and Dropout Base with Agar (DOBA) is a complete yeast nitrogen base with a carbon source. A complete supplemented synthetic defined medium is easily made by mixing DOB and Complete Supplement Mixture (CSM) which contains amino acids, adenine and uracil. CSM is also availble in Dropout formulations.|Packaging options include: Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Pre-poured Plates with agar, Ready-to-Use! |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!|
|Product Overview||For use with DOB/DOBA or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water.|
|Sterilization of Solutions||Autoclave at 121 °C for 15 minutes|
|Storage and Handling||Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|