DOB 2% galactose, 1% raffinose, 1 kg
DOB 2% Galactose, 1% Raffinose allows for preparation of a custom synthetic defined yeast media and includes a complete yeast nitrogen base (YNB) composed of buffering salts, vitamins and trace elements, with Galactose and Raffinose as the carbon source.
DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Dropout Base Medium |S.cerevisiae Growth medium|
|Application Notes||DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.|
|Base Catalog Number||4025932|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Media Type||Yeast Media|
|Formulation Details||Contents/liter: 1.7 g YNB; 5 g Ammonium Sulfate; 20 g Galatose; 10 g Raffinose|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Pack Size||1 kg|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||5.0 ± 0.3|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in DOB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution.|
|Product Overview||DOB 2% Galactose, 1% Raffinose is a complete yeast nitrogen base with slower burning Galactose and Raffinose as the carbon source. A complete supplemented synthetic defined medium is easily made by Mix DOB and CSM, a complete supplement mixture containing amino acids, adenine and uracil, of your own amino acid formulation to make a custom synthetic defined yeast media.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in Water (36.7 g/L)|
|Storage and Handling||Store dry DOB Medium powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Typical Working Concentration||36.7g/liter|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|
|Grade||Molecular Biology Certified™|