GLYCEROL, 80% (STERILE SOLUTI

GLYCEROL, 80% (STERILE SOLUTI

Synonyms 1,2,3-Propanetriol; Glycerin

CAS Number: GLYCEROL 56-81-5; WATER 7732-18-5 Molecular Formula: HOCH2CH(OH)CH2OH Molecular Weight: 92.09

Beilstein Registry Number: 635685 EC Number: 200-289-5

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SKU 113055034-CF
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$62.55
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Description

Product Description

Sterile 80% Glycerol solution is less viscous and more convenient to pipette than 100% glycerol.

Application Notes

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It has been used in purification of bacteriophage λ particles by step gradients, calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNA, DEAE-dextranmediated transfection of DNA into cells, DNA sequencing reactions, and mediation of mispriming in PCR. The use of glycerol in improved focusing of alkaline pH protein samples in immobilized pH gradient (IPG) strips has been published. Studies of the effect of glycerol on separation of DNA fragments by capillary electrophoresis have been reported.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Specifications
SKU 113055034-CF
Alternate Names 1,2,3-Propanetriol; Glycerin
Application Notes Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It has been used in purification of bacteriophage λ particles by step gradients, calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNA, DEAE-dextranmediated transfection of DNA into cells, DNA sequencing reactions, and mediation of mispriming in PCR. The use of glycerol in improved focusing of alkaline pH protein samples in immobilized pH gradient (IPG) strips has been published. Studies of the effect of glycerol on separation of DNA fragments by capillary electrophoresis have been reported.
Auto Ignition 698 °F (Lit.)
Beilstein Registry Number 635685
Boiling Point 182 °C/20 mmHg (Lit.)
Cells Tested E. coli
Certifications Molecular Biology Certified™
Chemical Class Carbohydrates
Color APHA ≤10
Concentration 0.8
Media Type Bacterial Media
Density 1.25 g/mL (Lit.)
EC Number 200-289-5
Flash Point 160 °C/320 °F (Lit.)
Format solution
Formulation Details 800 mL of 100% ACS grade Glycerol is combined with 200 mL of H2O and mixed well. It is then aliquoted into P.C. bottles and autoclaved.
Grade Molecular Biology Certified Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Molecular Formula HOCH2CH(OH)CH2OH
Molecular Weight 92.09
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH 6.0-7.0 (10% solution)
Physical Appearance Solution
Presentation Bottle
Product Families Description MP Biomedicals offers high quality components for preparation of your own custom formulations for growth and maintenance of recombinant E. coli strains. Tryptones, yeast extracts, amino acids, salts, trace minerals, agars and other reagents are available. Choices in formulation allow for custom formulations from small research batches up to pilot quantities. |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!
Product Overview Easy to pipet; excellent for freezing strains.
Refractive Index n20/D 1.474
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Oncology
Solubility Miscible in water.
Sterility Sterile
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Pressure <1 mmHg (20 °C)