M9 MINIMAL MEDIUM SALTS (MANIA

M9 MINIMAL MEDIUM SALTS (MANIA

Synonyms M9 Minimal Media

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SKU 113037012-CF
Price:
$78.85
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Description

Product Description

M9 Minimal Salts are a commonly used base for preparing a defined synthetic medium for the growth of most E. coli host cells. The powder is reconstituted in sterile purified water, (or water with melted purified agar added.) Additional nutrients, such as glucose or other carbon source, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria.

Application Notes

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Specifications
SKU 113037012-CF
Application Notes M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation.
Cells Tested E. coli
Certifications Molecular Biology Certified™
Components Contents/L of 5x solution: 33.9 g Na2HPO4 15 g KH2PO4 2.5 g NaCl, 5 g NH4Cl
Media Type Bacterial Media
Format powder
Formulation Details 33.9 g Na2HPO4; 15 g KH2PO4; 2.5 g NaCl; 5 g NH4Cl = 56.4 g/L of a 5x solution.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is: 6.5 - 7.2
Physical Appearance Powder
Preparation Method Media Preparation:Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in M9 Minimal Medium Salt formulations. Chosen reagents are combined in the proper proportions according to the formulation listed in the Certificate of Analysis and thoroughly blended for a uniform distribution.
Presentation Jar
Product Families Description In addition to L-Broth/LB-Medium classic formulations for growth and maintenance of recombinant E. coli strains, MP Biomedicals also offer other nutritionally rich mediums. Formulations contain high quality mixtures of tryptones, yeast extracts, salts and agars. |Choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!
Product Overview The Maniatis Formulation of M9 Minimal Media Salts consist of a single powder that is reconstituted to make a 5x stock solution for making M9 Growth Media. This is more a more convenient method as it eliminates the need to make two separate stocks.The powder is added to is water to make a sterile 5X concentrated solution and diluted to 1X before use with sterile water (or water plus melted, purified agar). Additional nutrients, such as glucose, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria.
Recommended Replacement For 113035022
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Oncology
Solubility Soluble in water
Sterilization of Solutions A 56.4 g sample of M9 Minimal Salts was dissolved in water to 1 liter, the pH adjusted to 7.4, if needed, and the solution autoclaved to make a sterile 5X concentrate.
Storage and Handling Store M9 Minimal Medium Salts at ambient temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.