L-BROTH (POWDER)

L-BROTH (POWDER)

Synonyms Luria Broth Base

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SKU 113001032-CF
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$114.50
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Description

Product Description

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains.

Application Notes

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation

Specifications
SKU 113001032-CF
Alternate Names Luria Broth Base
Application Notes L-Broth is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Cells Tested E. coli
Certifications Molecular Biology Certified™
Clarity NTU Clear once disolved in water
Media Type Bacterial Media
Format powder
Formulation Typically contain 10 g Tryptone; 5 g Yeast Extract; 0.5 g NaCl. Small quantities of biological buffers have been added for proper pH adjustment.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.9 ± 0.5 at 25 °C. Please note that the pH of L-Broth Medium liquid increases as the temperature of the solution decreases.
Physical Appearance Powder
Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in L-Broth Medium formulations. Chosen reagents are combined in the proper proportions according to the L-Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Presentation Jar
Product Families Description L-Broth/LB-Medium is a classic formulation of nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains and is still widely used today. Typical LB formulations contain 10 g of tryptone and 5 g of yeast extract per liter, and can vary in salt concentration from 0.5 g to 10 g per liter. Low or no salt formulations are mainly used when working with phage P1 where CaCl2 is used instead of NaCl to facilitate absorption of the phage into the cell. For plasmid growth, 5 or 10 g of NaCl works well. MP Biomedicals offers three forms of this traditional medium: - LB Medium contains 10 g NaCl per liter - Lennox LB Medium contains 5 g NaCl per liter - L-Broth contains 0.5 g NaCl per liter |Other choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!
Product Overview L-Broth, a common bacterial growth medium for E.coli cells, is widely used in molecular biology. The two main components of LB media are Tryptone and Yeast Extract. Tryptone is used in a concentration of 10 gram / liter and Yeast Extract in a concentration of 5 gram / liter. L-Broth is the low salt (NaCl) LB medium type with 0.5 gram /liter.
Recommended Replacement For CIRCLEGROW® Broth
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology
Solubility Soluble in water (16 g/L).
Storage and Handling Store dry L-Broth at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Typical Working Concentration 16 g/L
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.