RapidPure RNA Plant Kit

RapidPure RNA Plant Kit

Synonyms Plant RNA Isolation Kit

SKU 112722050
Price:
$410.20
In Stock
Request a quote
Description

Product Description

The RapidPURE RNA Plant Kit is designed to isolate and purify high quality total RNA from plant cells, plant tissues and filamentous fungi in a spin filter format. Special buffer conditionsguarantee an efficient lysis of the starting material and a simultaneous inactivation of endogenous RNases.

Application Notes

Depending on the expected yield or the needed concentration, total RNA can be eluted with different volumes of RNA Elution Buffer. A lower volume of RNA Elution Buffer will increase the concentration of RNA and a higher volume of RNA Elution Buffer will lead to an increased yield but lower concentration of total RNA. The minimum of Elution Buffer R should be 30 μl.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.

Specifications
SKU 112722050
Alternate Names Plant RNA Isolation Kit
Application Notes Depending on the expected yield or the needed concentration, total RNA can be eluted with different volumes of RNA Elution Buffer. A lower volume of RNA Elution Buffer will increase the concentration of RNA and a higher volume of RNA Elution Buffer will lead to an increased yield but lower concentration of total RNA. The minimum of Elution Buffer R should be 30 μl.
Capacity up to 100 mg or 1 x 107 cells of plant material or filamentous fungi
Cells Tested Allium cepa |Arabidopsis thaliana |Ascomyceten |Brassica oleracea |Casuarina (Australian tree) |Fusarium avenaceum |Gossypium spec. |Lycopersicon esculentum |Malus sylvestris |Mangifera indica |Nicotiana tabacum |Oryza sativa |Picea abies |Rosa hybrida |Solanaceae |Vitis vinifera | Zea mays |Actinidia deliciosa |Beta vulgaris |Brassica napus |Chlorophyceae |Fungi |Hordeum vulgare |Solanum tuberosum
Components Lysis Solution PS |Lysis Solution PH |RNA Wash Buffer 1 |RNA Wash Buffer 2 |RNA Elution Buffer |Prefilter |RNA Spin Filter Set |2.0 ml Collection Tubes |1.5 ml Elution Tubes |Manual
Format Chemical lysis
Applications Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.
Kit or Assay Type Nucleic Acid Purification Kits
Materials Required Not Supplied Microcentrifuge |96 - 100 % ethanol |Sterile RNase-free pipet tips |DTT or β-Mercaptoethanol
Molecule Enriched Total RNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution| Column
Presentation Kit
Product Overview The RapidPURE™ RNA Plant Kit is designed to isolate and purify high quality total RNA from plant cells, plant tissues and filamentous fungi in a spin filter format. Special buffer conditions guarantee an efficient lysis of the starting material and a simultaneous inactivation of endogenous RNases. Genomic DNA is separated from the total RNA by binding to specially optimized mineral carrier nanoparticles included in the Lysis Buffer. DNase-digestion to remove contaminating genomic DNA is usually not necessary. The RapidPURE™ RNA Plant Kit is the ideal tool for reliable and fast manual isolation and purification of high quality total RNA from up to 100 mg or 1 x 107 cells of plant material or filamentous fungi. The resulting purified RNA is ready to use. With the RapidPURE™ RNA Plant Kit procedure, all RNA molecules longer than 200 nucleotides are purified. The procedure provides enrichment for mRNA since most RNAs <200 nucleotides are selectively excluded. The size distribution of the purified RNA is comparable to that obtained by centrifugation through a CsCl cushion, where small RNAs do not sediment very efficiently. A separate protocol procedure allows for the isolation of RNA and intracellular proteins from the same sample.
Recommended Alternate FastRNA™ Pro GREEN Kit
Recommended Replacement For 116045050
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology
Safety Symbol GHS07 |GHS05 |GHS02 |GHS08
Sample Type plant cells; plant tissues and filamentous fungi
Sample Volume ≤ 100 mg
Storage and Handling Store at Room temperature (15-30 °C).
Trade Name RapidPURE™
Unit Definition ≤ 80 µg total RNA from up to 100 mg or 1 x 107 cells of plant material or filamentous fungi
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.