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RapidPure RNA Tissue Kit

RapidPure RNA Tissue Kit

Synonyms Tissue RNA Isolation Kit

Packaging size: 50 Preps

SKU 112721050
Price:
$372.05
In Stock
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Description

Product Description

The RapidPURETM RNA Tissue Kit is designed to isolate and purify high quality total RNA from small amounts of various human and animal tissues (e.g. muscle, liver, heart, and brain), tissue sections from lung, spleen, or kidney, and paraffin embedded tissue samples. The kit can also be used for simultaneous isolation of total RNA and proteins as well as for RNA cleanup in trizol phases.

Application Notes

Depending on the expected yield or the needed concentration, total RNA can be eluted with different volumes of RNA Elution Buffer. A lower volume of RNA Elution Buffer will increase the concentration of RNA and a higher volume of RNA Elution Buffer will lead to an increased yield but a lower concentration of total RNA. The minimum of RNA Elution Buffer should be 30 μl.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.

Specifications
SKU 112721050
Application Notes Depending on the expected yield or the needed concentration, total RNA can be eluted with different volumes of RNA Elution Buffer. A lower volume of RNA Elution Buffer will increase the concentration of RNA and a higher volume of RNA Elution Buffer will lead to an increased yield but a lower concentration of total RNA. The minimum of RNA Elution Buffer should be 30 μl.
Capacity up to 20 mg fresh or frozen tissue
Cells Tested muscle |liver |heart |brain |lung |spleen |kidney
Components Lysis Solution RT |RNAWash Buffer 1 |RNAWash Buffer 2 |RNA Elution Buffer |RNA Spin Filter Set |2.0 ml Collection Tubes |Spin Filter Catch Tubes |1.5 ml Elution Tubes |Manual
Format Chemical lysis
Applications Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.
Kit or Assay Type Nucleic Acid Purification Kits
Materials Required Not Supplied Microcentrifuge |96 - 100 % ethanol |Sterile RNase-free pipet tips |DTT or β-Mercaptoethanol
Molecule Enriched Total RNA
Pack Size 50 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution| Column
Presentation Kit
Product Overview The RapidPURE™ RNA Tissue Kit is designed to isolate and purify high quality total RNA from small amounts of various human and animal tissues (e.g. muscle, liver, heart, and brain), tissue sections from lung, spleen, or kidney, and paraffin embedded tissue samples. The kit can also be used for simultaneous isolation of total RNA and proteins as well as for RNA cleanup in trizol phases. Multiple samples can be processed simultaneously in less than 1 hour. Special buffer conditions guarantee an efficient lysis of the starting material and a simultaneous inactivation of endogenous RNases. Genomic DNA is separated from the total RNA by binding to specially optimized mineral carrier nanoparticles included in the Lysis Buffer. DNase-digestion to remove contaminating genomic DNA is usually not necessary. A specialized buffering system allows RNA species of sizes down to 200 bases to bind to the Spin Filter membrane. The RapidPURE™ RNA Tissue Kit is the ideal tool for reliable and fast manual isolation and purification of high quality total RNA from up to 20 mg fresh or frozen tissue sample in a spin filter format. The purified RNA is ready to use.
Recommended Alternate FastRNA™ Pro GREEN Kit
Recommended Replacement For 116045050
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Oncology
Safety Symbol GHS07 |GHS05 |GHS02 |GHS08
Sample Type human and animal tissues (e.g. muscle; liver; heart; and brain); tissue sections from lung; spleen; or kidney; and paraffin embedded tissue samples
Sample Volume ≤ 20 mg
Storage and Handling Store at Room temperature (15-30 °C).
Unit Definition ≤ 80 µg total RNA from up to 20 mg fresh or frozen tissue
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.