RapidPure DNA Plant Kit

RapidPure DNA Plant Kit

Synonyms Plant DNA Isolation Kit

Packaging size: 50 Preps

SKU 112712050
Price:
$246.35
In Stock
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Description

Product Description

The RapidPURE™ DNA Plant Kit is designed to isolate and purify total DNA from fresh, frozen or lyophilized plant material (such as leaves, roots, fruits or seeds) and also from a wide variety of food samples from plant origin (fresh, frozen or dried material). The RapidPURE™ DNA Plant Kit is the ideal tool for a rapid and efficient isolation of high quality genomic DNA from up to 100 mg plant material

Application Notes

Depending on the expected yield of genomic DNA, the DNA can also be eluted with a lower or a higher volume of Elution Buffer. The minimum elution volume is 50 μl. If quite large amount of DNA is expected, the volume of elution can be increased (100-200 μl). To maximize the final yield we recommend a second elution step with an equal volume of Elution Buffer.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.

Specifications
SKU 112712050
Alternate Names Plant DNA Isolation Kit
Application Notes Depending on the expected yield of genomic DNA, the DNA can also be eluted with a lower or a higher volume of Elution Buffer. The minimum elution volume is 50 μl. If quite large amount of DNA is expected, the volume of elution can be increased (100-200 μl). To maximize the final yield we recommend a second elution step with an equal volume of Elution Buffer.
Capacity up to 100 mg plant tissue
Components Plant Lysis Buffer |Binding Buffer |Proteinase K |Wash Buffer 1 |Wash Buffer 2 |Elution Buffer |Prefilter |Spin Filter |2.0 ml Collection Tubes |1.5 ml Collection Tubes |Manual
Format Chemical lysis
Applications Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.
Kit or Assay Type Nucleic Acid Purification Kits
Materials Required Not Supplied Microcentrifuge |Thermomixer or equivalent shaking heating block (for 65°C) |Vortex |Reaction tubes (1.5 ml) |dd H2O |Isopropanol |96 - 100 % ethanol |RNase A (10 mg/ ml - optional)
Molecule Enriched DNA
Pack Size 50 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution| Column
Presentation Kit
Product Overview The non-chaotropic buffer compositions used in RapidPure™ DNA Kits provide a chemically more gentle environment for nucleic acids as compared to buffers based on chaotropic salts such as the frequently used guanidium salts. As a consequence the DNA purified with RapidPure DNA Kits suffers less from chemical degradation and often provides more intact DNA. In applications requiring a maximum of intact DNA this is of crucial importance for an optimum assay performance. Using non-chaotropic chemistry the DNA bands are broader compared to the DNA from the chaotropic purification protocol. Furthermore, the lower edge of the bands are more diffuse indicating the presence of a continuum of shorter DNA fragments using a chaotropic chemistry.
Recommended Alternate FastDNA™ SPIN Kit
Recommended Replacement For 116540600
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS07 |GHS05 |GHS02 |GHS08
Sample Type fresh; frozen or lyophilized plant material (such as leaves; roots; fruits or seeds) and also from a wide variety of food samples from plant origin (fresh; frozen or dried material)
Sample Volume ≤ 100 mg
Storage and Handling Store at Room temperature (15-30 °C).
Trade Name RapidPURE™
Unit Definition ≤ 50 µg DNA from 100 mg plant tissue
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.