RapidPure DNA Plant Kit
The RapidPURE™ DNA Plant Kit is designed to isolate and purify total DNA from fresh, frozen or lyophilized plant material (such as leaves, roots, fruits or seeds) and also from a wide variety of food samples from plant origin (fresh, frozen or dried material). The RapidPURE™ DNA Plant Kit is the ideal tool for a rapid and efficient isolation of high quality genomic DNA from up to 100 mg plant material
Depending on the expected yield of genomic DNA, the DNA can also be eluted with a lower or a higher volume of Elution Buffer. The minimum elution volume is 50 μl. If quite large amount of DNA is expected, the volume of elution can be increased (100-200 μl). To maximize the final yield we recommend a second elution step with an equal volume of Elution Buffer.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.
|Alternate Names||Plant DNA Isolation Kit|
|Application Notes||Depending on the expected yield of genomic DNA, the DNA can also be eluted with a lower or a higher volume of Elution Buffer. The minimum elution volume is 50 μl. If quite large amount of DNA is expected, the volume of elution can be increased (100-200 μl). To maximize the final yield we recommend a second elution step with an equal volume of Elution Buffer.|
|Capacity||up to 100 mg plant tissue|
|Components||Plant Lysis Buffer |Binding Buffer |Proteinase K |Wash Buffer 1 |Wash Buffer 2 |Elution Buffer |Prefilter |Spin Filter |2.0 ml Collection Tubes |1.5 ml Collection Tubes |Manual|
|Applications||Nucleic Acid Isolation & Purification |PCR |qPCR |Sequencing (NGS) |Microarray |RFLP |Southern Blot Analysis.|
|Kit or Assay Type||Nucleic Acid Purification Kits|
|Materials Required Not Supplied||Microcentrifuge |Thermomixer or equivalent shaking heating block (for 65°C) |Vortex |Reaction tubes (1.5 ml) |dd H2O |Isopropanol |96 - 100 % ethanol |RNase A (10 mg/ ml - optional)|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Solution| Column|
|Product Overview||The non-chaotropic buffer compositions used in RapidPure™ DNA Kits provide a chemically more gentle environment for nucleic acids as compared to buffers based on chaotropic salts such as the frequently used guanidium salts. As a consequence the DNA purified with RapidPure DNA Kits suffers less from chemical degradation and often provides more intact DNA. In applications requiring a maximum of intact DNA this is of crucial importance for an optimum assay performance. Using non-chaotropic chemistry the DNA bands are broader compared to the DNA from the chaotropic purification protocol. Furthermore, the lower edge of the bands are more diffuse indicating the presence of a continuum of shorter DNA fragments using a chaotropic chemistry.|
|Recommended Alternate||FastDNA™ SPIN Kit|
|Recommended Replacement For||116540600|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology|
|Safety Symbol||GHS07 |GHS05 |GHS02 |GHS08|
|Sample Type||fresh; frozen or lyophilized plant material (such as leaves; roots; fruits or seeds) and also from a wide variety of food samples from plant origin (fresh; frozen or dried material)|
|Sample Volume||≤ 100 mg|
|Storage and Handling||Store at Room temperature (15-30 °C).|
|Unit Definition||≤ 50 µg DNA from 100 mg plant tissue|