Dino

MINIPREP EXPRESS MATRIX

MINIPREP EXPRESS MATRIX

Synonyms Plasmid Miniprep Purification Kit

Packaging size: 1250 Preps

SKU 112000200
Price:
$253.50
In Stock
Request a quote
Description

Product Description

MiniPrep™ Express Matrix provides the essential ingredient for making your “own kit” for doing large numbers of minipreps for rapid screening purposes that involve a restriction digest. This kit allows you to make the lysis reagents from the most commonly used methods.

Application Notes

Miniprep Express™ matrix is designed for rapid purification of plasmid DNA. It is ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Rapid preparation of plasmid DNA

Specifications
SKU 112000200
Application Notes Miniprep Express™ matrix is designed for rapid purification of plasmid DNA. It is ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.
Components MiniPrep Express™ Matrix, Protocols and recipes necessary for either alkaline lysis or boiling miniprep procedures.
Applications Rapid preparation of plasmid DNA
Kit or Assay Type Plasmid DNA isolation kit
Materials Required Not Supplied 80% ethanol | Hot plate or burner| Alkaline Lysis procedure: Reagents necessary to make Solutions I, II and III (protocol provided) | Boiling procedure: Reagents necessary to make STET solution and Lysozyme solution (protocol provided) | Any other preferred lysis or digest method is user provided.
Molecule Enriched DNA
Pack Size 1250 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution| Slurry
Presentation Kit
Product Overview The Miniprep Express™ is a truly cost effective plasmid miniprep. At 20 cents a prep, it is the frequent cloners ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR. The Miniprep Express™, which consists only of the Miniprep Express Matrix™, is designed to complement the alkaline lysis and boiling miniprep procedures. Simply add 400 μl Miniprep Express Matrix™ to the crude plasmid prep, wash, air dry and elute the DNA in 75 μl H2O or TE. Plasmid DNA purified by the Miniprep Express™ does not require the use of RNase since the majority of the bacterial RNA does not bind to the Miniprep Express Matrix™ and is lost in the supernate. The residual RNA, if any, that copurifies with the plasmid DNA does not interfere with PCR, restriction enzyme analysis and DNA visualization on ethidium bromide stained gels. The Miniprep Express™ allows the researcher to screen 30 - 60 minipreps in 1 - 2 hours without using any dedicated equipment. Once the desired clones are identified, MP Bio offers a complete family of purification kits to isolate GENECLEAN quality, 2x CsCl quality, plasmid DNA in any desirable scale: μg - mg amounts.
Recommended Alternate RapidPURE™ Plasmid Mini Kit
Recommended Replacement For 112066200 | 112066400 | 112066600
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Sample Throughput 30 - 60 minipreps in 1 - 2 hours
Sample Type Plasmid Prep
Sample Volume 1 ml of overnight culture
Storage and Handling Store at Room Temperature (15-30 °C).
Unit Definition Yields 1 - 5 μg of High Copy Number Vector DNA Per 1 ml overnight culture typical
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.