Columbia Agar Base

Columbia Agar Base

Packaging size: 10 L

SKU 091003122
Price:
$25.80
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Description

Product Description

Columbia Agar Base is a highly nutritive general purpose medium for the cultivation of fastidious organisms, especially when supplemented with plain or chocolated blood. Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.

Application Notes

Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.

Key Applications

Bacteria Cell Culture | Mammalian Cell Culture

Specifications
SKU 091003122
Application Notes Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.
Formulation Powder | Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2
Applications Bacteria Cell Culture | Mammalian Cell Culture
Pack Size 10 L
pH 6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)
Physical Appearance Orange Powder
Preparation Method Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121 °C (15 lbs. psi) for 15 minutes. Cool to 45-50 °C and add 5-10% sterile defibrinated sheep or rabbit blood if desired.
Presentation Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2 | Powder
Storage and Handling Store at +4°C.