Human Albumin Fraction V
Product Description
This human protein is prepared by a modification of the Cohn procedure. Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis). Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.
Application Notes
Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis).
Key Applications
Immunoassays | Mammalian Cell Culture
SKU | 08823022 |
Alternate Names | HAS |
Application Notes | Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis). |
Concentration | > 98% |
EC Number | 274-272-6 |
Formulation | Powder |Fraction V |
Host | Human |
Isoelectric Point | (G/2 = 0.15) 4.7 (Lit.) |
Applications | Immunoassays | Mammalian Cell Culture |
Pack Size | 10 g |
pH | 6.87 |
Physical Appearance | Powder |
Preparation Method | This human protein is prepared by a modification of the Cohn procedure. |
Presentation | powder |
Protein or Enzyme Type | Albumins |
Purity | 0,96 |
Solubility | Soluble in water |
Storage and Handling | Store at 2 - 8°C. For long-term storage; aliquot and freeze at 20°C. Avoid repeated freeze/thaw cycles. |
Trade Name | ImmunO™ |