Bovine Albumin Fraction V (Protease Free)
Description
Product Description
Albumin is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Application Notes
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Key Applications
Immunoblot | ELISA |Mammalian Cell Culture |ADME| Standards
Specifications
| SKU | 0882045-CF |
| Alternate Names | BSA; Bovine albumin |
| Application Notes | Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal. |
| Concentration | Protein: 99.0% |
| EC Number | 232-936-2 |
| Formulation Details | Powder |Protease Free |
| Grade | Protease Free |
| Host | Bovine |
| Isoelectric Point | At 25 °C: Fatty Acid Depleted- 5.3, Endogenous Material- 4.7; 4.9 (Lit.) |
| Applications | Immunoblot | ELISA |Mammalian Cell Culture |ADME| Standards |
| pH | 7.0 (10% aqueous solution); 6.9 (saline) |
| Preparation Method | Plasma undergoes salt fractionation using caprylic acid (sodium salt), heating at 65 °C for three hours; alcohol precipitation followed by an acetone wash resulting in a Fraction V powder |
| Presentation | Powder |Protease Free |
| Product Families Description | Albumin is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group. |
| Protein or Enzyme Type | Albumins |
| Purity | 99.0% |
| Solubility | Soluble in 40 mg/mL, H<sub>2</sub>O. |
| Source | Bovine blood |
| Storage and Handling | Store at 2-8°C. |
| Trade Name | ImmunO™ |