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Pure Pokeweed Mitogen (Phytolacca Americana)

Pure Pokeweed Mitogen (Phytolacca Americana)

Synonyms PWM; Pokeweed mitogen; Lectin from Phytolacca americana(pokeweed)

Molecular Weight: 32 kDa (sedimentation equilbrium) (Lit.)

Packaging size: 1 mg

SKU 08780341
Price:
$144.45
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Description

Product Description

Product lectin is pure Phytolacca americana from Pokeweed. Pure pokeweed mitogen is used in mitogenic assays. It has variety of applications in vitro like blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, lymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions. Pokeweed mitogen has specificity for sugar chains containing a di-N-Acetylchitobiose moiety such as is found on "band-3 glycoprotein" from human erythrocytes. Di-N-acetylchitobiose and tri-N-acetylchitobiose inhibit agglutination activity of the lectin.

Application Notes

Pure pokeweed mitogen is used in mitogenic assays. It has variety of applications in vitro like blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, lymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.

Key Applications

Immunoassays | Mitogenic assay

Specifications
SKU 08780341
Application Notes Pure pokeweed mitogen is used in mitogenic assays. It has variety of applications in vitro like blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, lymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.
Formulation Details Lyophilized
Applications Immunoassays | Mitogenic assay
Molecular Weight 32 kDa (sedimentation equilbrium) (Lit.)
Pack Size 1 mg
Physical Appearance Lyophilized powder
Presentation lyophilized
Product Overview Pokeweed mitogen has specificity for sugar chains containing a di-N-Acetylchitobiose moiety such as is found on "band-3 glycoprotein" from human erythrocytes. Di-N-acetylchitobiose and tri-N-acetylchitobiose inhibit agglutination activity of the lectin.
Protein or Enzyme Type Other Proteins, Enzymes Peptides
Solubility Soluble in phosphate buffered saline (1 mg/mL).
Source Phytolacca americana(pokeweed)
Specific Activity Less than 0.5 mg/mL will agglutinate neuraminidase treated human red blood cells.
Storage and Handling Store lyophilized product at 2-8°C. After reconstitution; aliquot and store at -20°C. Avoid Freeze/Thaw cycles.