Tubulin, Purified from Bovine Brain

Tubulin, Purified from Bovine Brain

Packaging size: 1 mg

SKU 08771121
Price:
$550.00
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Description

Product Description

Purified bovine brain tubulin protein supplied in liquid form at 10 mg/mL in 80 mM PIPES buffer containing 1 mM EGTA, 1 mM GTP, pH 6.8 and 10% glycerol. It is a heterodimer composed of α- and β- tubulin of 55 kDa (molecular weight ≅110 kDa). Polymerization occurs forming microtubules of 25 nm in diameter and average 1650 heterodimers per µm in length. One unit of tubulin is defined as 5.0 mg of purified protein (as determined by the method of Bradford: Bradford, M., Analytical Biochemistry 72, 248, 1976).

Usage Statement

Tubulin protein is a labile molecule that loses activity within hours of being thawed. When handling tubulin, it is strongly recommended to follow the instructions detailed herein. Even perceived slight differences in handling can have a significant impact on product quality. If not used immediately, aliquot reconstituted material. Must be snap frozen in liquid nitrogen prior to storage at -80°C. Failure to do so will result in a significant loss of activity. To thaw a frozen aliquot of tubulin, place in a room temperature water bath. Once it is completely thawed place on ice.

Specifications
SKU 08771121
Base Catalog Number 771121
Format liquid
Pack Size 1 mg
Preparation Method One unit of tubulin in G-PEM buffer pH 6.8 plus 10% glycerol was incubated at 35°C in a cuvette. The optical density at 340 nm was taken at time intervals. A value of 1.0 OD unit indicates that >97% of the tubulin has polymerized. The polymerization reaction was performed in duplicate.
Purity 99% (By SDS-PAGE). Single band at 55 kDa.
Research Areas Cell Structure, Cell Biology
Specificity When tubulin at a concentration of 1.0 unit/mL (in G-PEM buffer plus 10% glycerol [v/v]) is incubated at 35°C for 30 minutes an increase in optical density (OD340nm) from 0.0 to 1.0 is observed. This corresponds to a total microtubule (MT) polymer mass of >4.8 mg/mL. The mean length of the microtubule population under these conditions is approximately 10 µm as measured by immunoflourescence microscopy and the average number of MTs per ml is 1.0 x 10<sup>12</sup>.
Storage and Handling Store at -80°C
Usage Statement Tubulin protein is a labile molecule that loses activity within hours of being thawed. When handling tubulin, it is strongly recommended to follow the instructions detailed herein. Even perceived slight differences in handling can have a significant impact on product quality. If not used immediately, aliquot reconstituted material. Must be snap frozen in liquid nitrogen prior to storage at -80°C. Failure to do so will result in a significant loss of activity. To thaw a frozen aliquot of tubulin, place in a room temperature water bath. Once it is completely thawed place on ice.