Mouse Anti-Glial Fibrillary Acidic Protein (GFAP) Monoclonal

Mouse Anti-Glial Fibrillary Acidic Protein (GFAP) Monoclonal

Synonyms Anti-GFAP; Monoclonal Anti-Glial Fibrillary Acidic Protein

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SKU 0869110-CF
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$287.50
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Description

Product Description

Product is a mouse monoclonal antibody, isotype IgG1, that reacts with Glial Fibrillary Acidic Protein.

Application Notes

Mouse Anti - Glial Fibrillary Acidic Protein is used in immunohistochemistry. It is designed for the specific and qualitative localization of GFAP in formalin-fixed paraffin-embedded tissue sections. This antibody stains glial cells (Bergmann glia) and astrocytes. It has been tested for immunohistochemistry in human, pig, and rat. It is also suitable for microarray.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Immunoassays | Immunohistochemistry | Microarray

Specifications
SKU 0869110-CF
Alternate Names Anti-GFAP; Monoclonal Anti-Glial Fibrillary Acidic Protein
Antibody Category Monoclonal
Antibody Type Monoclonal Antibody
Application Notes Mouse Anti - Glial Fibrillary Acidic Protein is used in immunohistochemistry. It is designed for the specific and qualitative localization of GFAP in formalin-fixed paraffin-embedded tissue sections. This antibody stains glial cells (Bergmann glia) and astrocytes. It has been tested for immunohistochemistry in human, pig, and rat. It is also suitable for microarray.
Clone Name GA-5
Concentration Titer- 1:300
Conjugate Unconjugated
Formulation Liquid
Gene ID Mouse: (Gfap 14580)
Host Mouse
Immunogen GFAP isolated from porcine spinal cord
Isotype IgG1
Applications Immunoassays | Immunohistochemistry | Microarray
Preparation Method The liquid antibody (from ascites) is in phosphate buffered saline, pH 7.6, with 1% BSA and 0.09% sodium azide as a preservative. The original immunogen was produced by the fusion of mouse myeloma cells and mouse spleen cells immunized with purified porcine GFAP. By use of well defined GFAP fragments, the antibody was shown to react with the carboxyterminal Cys II fragment. It appears to be broadly cross-reactive among species. Immunoblots on extracts of the glioma cell line U333 CG/U343MG specifically visualize a single 51 kDa band. The antibody was prepared using GFAP isolated from porcine spinal cord as the immunogen.
Presentation Storage Buffer: The liquid antibody (from ascites) is in phosphate buffered saline; pH 7.6; with 1% BSA and 0.09% sodium azide as a preservative.
Product Families Description Product is a mouse monoclonal antibody, isotype IgG1, that reacts with Glial Fibrillary Acidic Protein.
Research Areas Cytoskeletal Signaling||Neural Stem Cells||Cell Biology
Solubility Soluble in water
Species Reactivity Pig; Rat; Human
Specificity The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes; Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.
Storage and Handling Store product at 2-8°C. Do not Freeze.
Trade Name ImmunO™
Typical Working Concentration Immunohistochemical use: 1:50 to 1:100.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.