Silver Enhancing Kit (For Blotting)
Silver Enhancing Kit is an easy to use and sensitive method for enhancement of immunogold labelled antigens on a solid support. It depends on the reduction of silver from solution in the presence of a developer by the gold label attached to proteins/nucleic acids mounted on a solid phase for direct viewing by eyes. The components of the solutions have been selected to allow good control over time of development with minimal background. Because of its relative insensitivity to light, the enhancement may be monitored in normal room lighting during development. The specimens need no fixing after development but may be thoroughly washed in tap water. The development operates at pH 7.4 to minimize the difficulties experienced with other enhancing methods where very low pH sometimes causes the extraction of labelled proteins from the specimen.
- Enhance the sensitivity of gold signal to 1 picogram
- High signal resolution with low background
- Slow, controlled development of silver signal
- Light-stable under ordinary laboratory lighting
Immu-Mark™ silver enhancing kit is used for amplifying the signal from gold particles attached to proteins or antigens immobilized on membranes. By intensifying the gold probe signal, protein levels in the 1 picogram range are easily detectable.
Immunoassays | Immunoblot
|Application Notes||Immu-Mark™ silver enhancing kit is used for amplifying the signal from gold particles attached to proteins or antigens immobilized on membranes. By intensifying the gold probe signal, protein levels in the 1 picogram range are easily detectable.|
|Base Catalog Number||678211|
|Components||Contains 1 x 250 mL Silver Enhancer Solution A (Initiator) and 1 x 250 mL, Silver Enhancer Solution B (Enhancer).|
|Applications||Immunoassays | Immunoblot|
|Pack Size||1 Kit|
|Product Overview||Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancing kit enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting. The kit is easy-to-use and comes with a simple-to-follow protocol. The kit reagents are formulated to provide rapid, intense staining with low background.|
|Storage and Handling||Store at 2-8°C. DO NOT FREEZE. Do not expose to extreme heat or light.|