Mouse IgG (Lyophilized)
Product Description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Mouse IgG is purified from normal mouse serum.
Application Notes
Mouse IgG is useful for structural and biological studies, for use as reference standards or as starting material for the preparation of immunogen. Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Immunoassays
| SKU | 0864146-CF |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Mouse IgG is useful for structural and biological studies, for use as reference standards or as starting material for the preparation of immunogen. Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors. |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized | Each vial contains 10 mg of lyophilized protein based on; E<sup>1%</sup><sup>280 nm</sup> = 14. This product was lyophilized from 0.01M sodium phosphate; 0.07M sodium chloride; pH 7.3. There is no preservative added. | It is supplied as purified immunogen in either Tris or phosphate buffered saline. |
| Host | Mouse |
| Applications | Immunoassays |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| Physical Appearance | Lyophilized powder |
| Preparation Method | This immunoglobulin was prepared from mouse serum using current chromatographic techniques. A quantitative protein analysis as well as immunoelectrophoresis has been applied to determine identity of the product. |
| Presentation | Each vial contains 10 mg of lyophilized protein based on; E<sup>1%</sup><sup>280 nm</sup> = 14. This product was lyophilized from 0.01M sodium phosphate; 0.07M sodium chloride; pH 7.3. There is no preservative added. |
| Product Families Description | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Mouse IgG is purified from normal mouse serum. |
| Purity | > 95% by SDS-PAGE |
| Solubility | Soluble in water |
| Specificity | By immunoelectrophoresis: Antibody vs. rabbit anti-mouse serum - shows one band. The product is mouse IgG with no trace of IgA; IgM; or non-immunoglobulin serum proteins detected. |
| Storage and Handling | Store the lyophilized product at 2 - 8°C. For long-term storage; after reconstitution; aliquot and store frozen at or below -20°C. Avoid repeated freeze/thaw cycles. |
| Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |