Rabbit Peroxidase Anti-Peroxidase (Pap)
Product is the lyophilized powder of rabbit peroxidase anti-peroxidase complexes (PAP) and buffer salts
Rabbit Peroxidase Anti-Peroxidase Soluble Complex is suitable for use in peroxidase enzymatic labeling experiments, intended for use in immunostaining of cells, tissues, and blots. It is also suitable for use in ELISA and other enzyme immunoassays.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | Peroxidase enzymatic labeling experiments | Immunohistochemistry
|Analysis Note||Rabbit PAP is tested for purity at 20 mg/mL with anti-rabbit serum and anti-horseradish peroxidase by immunoelectrophoresis; No traces of non-immunoglobulin rabbit serum components are detected.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Rabbit Peroxidase Anti-Peroxidase Soluble Complex is suitable for use in peroxidase enzymatic labeling experiments, intended for use in immunostaining of cells, tissues, and blots. It is also suitable for use in ELISA and other enzyme immunoassays.|
|Certifications||The product contains rabbit IgG complexed with peroxidase.|
|Applications||Immunoassays | Peroxidase enzymatic labeling experiments | Immunohistochemistry|
|Pack Size||5 mg|
|Physical Appearance||Lyophilized powder|
|Preparation Method||Prepared by the method of Stemberger. Antibodies to peroxidase are purified by immunoprecipitation with purified horseradish peroxidase (Rz>3.0) at the equivalence point. After extensive washing, the immunoprecipitate is solubilized at low pH. PAP complexes are made soluble by addition of excess peroxidase and neutralization and are purified from free peroxidase by repeated salt fractionation. The resulting PAP complexes are dialyzed into 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6, adjusted to standard protein concentration, filtered through a 0.22 μm filter, vialed and lyophilized. The product may contain preservative.|
|Recommended Replacement For||861242|
|Solubility||Soluble in water|
|Storage and Handling||Store lyophilized product at 2-8°C. For long term storage; aliquot and freeze reconstituted product at -20°C or lower. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use .|