Purified Rat IgG

Purified Rat IgG

Synonyms IgG from rat serum; Rat IgG

MDL Number: MFCD00164131

Packaging size: 20 mg

SKU 0855951
Price:
$206.95
In Stock
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Description

Product Description

Product is the lyophilized powder of rat IgG and buffer salts.

Application Notes

Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG. It is suitable for use as a positive control in antibody identification screening techniques.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis

Specifications
SKU 0855951
Alternate Names IgG from rat serum; Rat IgG
Analysis Note The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 20 mg of rat IgG is obtained upon reconstitution.
Antibody Type Polyclonal Antibody
Application Notes Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG. It is suitable for use as a positive control in antibody identification screening techniques.
Concentration Total Protein: 10.4 mg/mL; Volume: 2 mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rat
Isotype IgG
Applications Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis
MDL # MFCD00164131
Pack Size 20 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Lyophilized powder
Preparation Method Rat IgG is purified from pooled rat serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, and filtered through a 0.22 µm filter, vialed and lyophilized. No preservative is added.
Presentation lyophilized
Purity > 99%; lyophilized
Solubility Soluble in water
Source Rat
Storage and Handling Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -20°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.