Purified Rabbit IgG

Purified Rabbit IgG

Synonyms IgG from rabbit serum; Rabbit IgG

MDL Number: MFCD00165673

Packaging size: 50 mg

SKU 0855944
Price:
$206.20
In Stock
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Description

Product Description

Product is the lyophilized powder of rabbit IgG and buffer salts. Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.

Application Notes

Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis

Specifications
SKU 0855944
Alternate Names IgG from rabbit serum; Rabbit IgG
Analysis Note The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 50 mg of rabbit IgG is obtained upon reconstitution.
Antibody Type Polyclonal Antibody
Application Notes Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.
Concentration Total Protein: 18.2 mg/mL; Volume: 3 mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rabbit
Isotype IgG
Applications Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis
MDL # MFCD00165673
Pack Size 50 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Lyophilized powder
Preparation Method Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.
Presentation lyophilized
Product Families Description Product is the lyophilized powder of rabbit IgG and buffer salts.
Purity > 99%; lyophilized
Recommended Replacement For 0864147 | 0855904
Solubility Soluble in water
Source Rabbit
Storage and Handling Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -20°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.