Purified Human Transferrin
Product is the lyophilized powder of human transferrin and buffer salts. ransferrin is a glycoprotein with homologous N-terminal and C-terminal iron-binding domains. The N-terminal and C-terminal domains of this protein are globular moieties of about 330 amino acids. Each of these domains is divided into two sub-domains, with the iron- and anion-binding sites found within the intersubdomain cleft. The binding cleft opens with iron release and closes with iron binding. Transferrin binds iron with an association constant of approximately 1022 M-1. Ferric iron couples to transferrin only in the presence of an anion (usually carbonate) that serves as a bridging ligand between metal and protein, excluding water from the two coordination sites. Without the anion cofactor, iron binding to transferrin is negligible. In the presence of anions, ferric transferrin is resistant to all but the most potent chelators. The remaining four coordination sites are provided by the transferrin protein - a histidine nitrogen, an aspartic acid carboxylate oxygen, and two tyrosine phenolate oxygens. Available evidence suggests that anion-binding takes place prior to iron-binding. Iron release from transferrin involves protonation of the carbonate anion, loosening the metal-protein bond.
Transferrin delivers iron to cells in a physiologically safe and effective form.
Immunoassays | Iron binding Protein
|Application Notes||Transferrin delivers iron to cells in a physiologically safe and effective form.|
|Gene ID||Human (TF 7018)|
|Applications||Immunoassays | Iron binding Protein|
|Molecular Weight||Approximately 76-81 kDa (Lit.)|
|Pack Size||10 mg|
|Physical Appearance||Lyophilized powder|
|Preparation Method||Human transferrin is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.|
|Product Families Description||Product is the lyophilized powder of human transferrin and buffer salts.|
|Protein or Enzyme Type||Plasma Blood Proteins|
|Research Areas||Blood Proteins|
|Solubility||Soluble in water (50 mg/mL).|
|Storage and Handling||Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -20°C.|