Purified Human Serum IgA

Purified Human Serum IgA

Packaging size: 5 mg

SKU 0855906
Price:
$414.25
In Stock
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Description

Product Description

Product is the lyophilized powder of human serum IgA and buffer salts.

Application Notes

Purified human serum IgA is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays

Specifications
SKU 0855906
Analysis Note The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is human serum IgA; no trace of IgG, IgM or non-immunoglobulin serum proteins is detected. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 5 mg of human serum IgA is obtained upon reconstitution.
Antibody Type Polyclonal Antibody
Application Notes Purified human serum IgA is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Conjugate Unconjugated
Formulation Details Lyophilized
Host Human
Applications Immunoassays
Pack Size 5 mg
Physical Appearance Lyophilized powder
Preparation Method Human IgA is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation lyophilized
Purity > 99%
Solubility Soluble in water
Storage and Handling Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -20°C
Typical Working Concentration Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.