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Peroxidase-Conjugated Protein A

Peroxidase-Conjugated Protein A

Synonyms Protein A, HRP conjugate

Packaging size: 2 mg

SKU 0855901
Price:
$254.40
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Description

Product Description

Product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase. This product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase. Protein A is a component of the cell wall of Staphylococcus aureus (Cowan 1 Strain), and binds to the Fc portion of immunoglobulin molecules of most mammalian species. It is composed of a single polypeptide chain of molecular weight 42,000. Protein A binds IgG molecules and, in some cases, IgM and IgA.

Application Notes

Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. It is intended for use in cell and tissue staining, dotting and blotting, ELISA and other immunoassays. For each specific experiment, the product should be titered to maximize signal to background.

Key Applications

Immunoassays | Dot Blot | ELISA | Immunoblot | Immunohistochemistry

Specifications
SKU 0855901
Application Notes Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. It is intended for use in cell and tissue staining, dotting and blotting, ELISA and other immunoassays. For each specific experiment, the product should be titered to maximize signal to background.
Conjugate HRP
Formulation Details Purified
Applications Immunoassays | Dot Blot | ELISA | Immunoblot | Immunohistochemistry
Pack Size 2 mg
Preparation Method The product is in 10mM phosphate, 0.15M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol containing 0.1% proclin as preservative.
Presentation Liquid
Product Families Description Product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase.
Purity Immunoaffinity Chromatography 
Species Reactivity Binding Characteristics of Protein A: Human; Rabbit; Mouse; Sheep; Goat; Rat; Dog; Guinea pig; Pig; Cow
Storage and Handling Store unopened product at 2-8°C. Do not freeze. If accidentally frozen; centrifuge to clarify and precipitate before use.
Typical Working Concentration Suggested Trial Dilutions: 1:100 - 1:200; 1:8,000 - 1:16,000