Peroxidase-Conjugated Rabbit IgG Fraction To Swine IgG (Whole Molecule)

Peroxidase-Conjugated Rabbit IgG Fraction To Swine IgG (Whole Molecule)

Synonyms Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit

MDL Number: MFCD00162752

Packaging size: 2 mL

SKU 0855826
Price:
$260.40
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Description

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit F(ab')2 fragment to swine IgG (whole molecule) and buffer salts.

Application Notes

Product is used in Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. Also it is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.

Usage Statement

This product is intended FOR RESEARCH USE ONLY, not for use in diagnostic or therapeutic procedures involving humans or animals

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855826
Alternate Names Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with swine IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly rabbit IgG; no trace of albumin is detected
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Product is used in Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. Also it is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.
Concentration Total Protein: 19.0 mg/mL; Titer: 1:88;000
Conjugate HRP
Formulation Lyophilized
Host Rabbit
Immunogen Purified Pig IgG
Applications Immunohistochemistry | Immunoblotting |ELISA
MDL # MFCD00162752
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation lyophilized
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Swine; (Whole Molecule)
Specificity It shows reactivity to swine IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement This product is intended FOR RESEARCH USE ONLY, not for use in diagnostic or therapeutic procedures involving humans or animals