Peroxidase-Conjugated Sheep IgG Fraction To Rat Albumin

Peroxidase-Conjugated Sheep IgG Fraction To Rat Albumin

MDL Number: MFCD00162813

Packaging size: 2 mL

SKU 0855776
Price:
$210.15
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Description

Product Description

Peroxidase-conjugated sheep IgG fraction to rat albumin is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to rat albumin and buffer salts. IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG conjugated to a detectable substrate is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat immunoglobulins.

Application Notes

Peroxidase-conjugated sheep IgG fraction to rat albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Enzyme Immunoassays | Immunoblot

Specifications
SKU 0855776
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rat albumin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly sheep IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated sheep IgG fraction to rat albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.
Concentration Total Protein: 20.8 mg/mL Titer: 1:109;000
Conjugate HRP
Formulation Details Lyophilized
Host Sheep
Immunogen Purified rat IgG
Applications Immunoassays | ELISA | Enzyme Immunoassays | Immunoblot
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Presentation lyophilized
Purity Purified
Solubility Soluble in water
Species Reactivity Rat
Specificity It shows reactivity to rat albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1:8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.