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Peroxidase-Conjugated Goat Affinity Purified F(Ab')2 Fragment To Rabbit IgG (Whole Molecule)

Peroxidase-Conjugated Goat Affinity Purified F(Ab')2 Fragment To Rabbit IgG (Whole Molecule)

MDL Number: MFCD00162788

Packaging size: 1 mL

SKU 0855693
Price:
$199.10
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Description

Product Description

Peroxidase-conjugated goat affinity purified F(ab')2 fragment to Rabbit IgG (Whole Molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified F(ab')2 fragments to rabbit IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855693
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as a standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rabbit IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The fragment is goat F(ab')<sub>2</sub>; no trace of albumin, whole molecule IgG or Fc is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 1.0 mg/mL; Titer: 1:20;000
Conjugate HRP
Formulation Details Purified
Host Goat
Immunogen Purified rabbit IgG
Applications Immunohistochemistry | Immunoblotting |ELISA
Pack Size 1 mL
Physical Appearance Liquid
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added.
Presentation Liquid
Product Families Description Peroxidase-conjugated goat affinity purified F(ab')2 fragment to Rabbit IgG (Whole Molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified F(ab')2 fragments to rabbit IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rabbit; (Whole Molecule)
Specificity Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Typical Working Concentration Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution. Peroxidase-conjugated goat anti-rabbit IgG antibody was used for wester blot analysis of yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.