Dino

Peroxidase-Conjugated Goat Affinity Purified Antibody To Rabbit IgG Fc

Peroxidase-Conjugated Goat Affinity Purified Antibody To Rabbit IgG Fc

Packaging size: 2 mL

SKU 0855690
Price:
$214.65
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Description

Product Description

Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG Fc and buffer salts.

Application Notes

This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855690
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Concentration Total Protein: 0.6 mg/ml Titer: 1:23;000
Conjugate HRP
Formulation Details Purified
Host Goat
Applications Immunohistochemistry | Immunoblotting |ELISA
Pack Size 2 mL
Physical Appearance Liquid
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.
Presentation Liquid
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rabbit; IgG Fc
Specificity It shows reactivity to rabbit Fc and IgG; cross-reactivity to other species may exist. Antibody activity to F(ab')<sub>2</sub>; IgA; IgM; or non-immunoglobulin serum proteins is not present.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 -1: 4,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.