Fluorescein-Conjugated Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

Fluorescein-Conjugated Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

MDL Number: MFCD00162786

Packaging size: 2 mg

SKU 0855662
Price:
$176.65
In Stock
Request a quote
Description

Product Description

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855662
Analysis Note The total protein is measured by absorbance (A280nm); F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the affinity purified antibody is standardized by equivalence-point precipitation. The affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors).
Concentration Total Protein: 1.0 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate FITC
Formulation Purified
Host Goat
Immunogen Purified rabbit IgG
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
MDL # MFCD00162786
Pack Size 2 mg
Physical Appearance Liquid
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.
Presentation Liquid
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rabbit; (Whole Molecule)
Specificity Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Trade Name Cappel™
Typical Working Concentration Anti-Rabbit IgG (whole molecule)-FITC antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:160. A minimum working dilution of 1:160 may be used on formalin-fixed, paraffin-embedded human tonsil sections and Anti-Human IgG as the primary antibody. This antibody was used for immunefluorescence of Alfalfa root and leaf sections stained with anti-Medicago sativa GSK-3-like kinase primary antibody. For immunostaining of human testis cryosections, a dilution of 1:200 was used with N-cadherin primary antibody.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.