Peroxidase-Conjugated Goat Affinity Purified Antibody To Mouse IgG (Whole Molecule)

Peroxidase-Conjugated Goat Affinity Purified Antibody To Mouse IgG (Whole Molecule)

MDL Number: MFCD00162644

Packaging size: 2 mL

SKU 0855563
Price:
$163.35
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Description

Product Description

Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-conjugated goat affinity purified antibody to mouse IgG is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.) Anti-mouse IgG (whole molecule)-peroxidase antibody can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855563
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as a standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated goat affinity purified antibody to mouse IgG is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.) Anti-mouse IgG (whole molecule)-peroxidase antibody can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.
Concentration Total Protein: 0.7 mg/ml Titer: 1:48;000
Conjugate HRP
Formulation Liquid
Host Goat
Immunogen Purified mouse IgG
Applications Immunohistochemistry | Immunoblotting |ELISA
MDL # MFCD00162644
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added.
Presentation Liquid
Product Families Description Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Recommended Replacement For 0855565 | 0855572 | 0868396
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity Product shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.