Dino

Fluorescein-Conjugated Goat Affinity Purified F(Ab')2 Fragment To Mouse Immunoglobulins (IgG, IgA, IgM)

Fluorescein-Conjugated Goat Affinity Purified F(Ab')2 Fragment To Mouse Immunoglobulins (IgG, IgA, IgM)

Packaging size: 2 mg

SKU 0855526
Price:
$239.20
In Stock
Request a quote
Description

Product Description

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')2 fragments to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855526
Analysis Note The total protein is measured by absorbance (A)<sub>280 nm</sub>); F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the affinity purified F(ab')<sub>2</sub> fragments is standardized with an in-house control by immunoelectrophoresis. The affinity purified F(ab')<sub>2</sub> fragment is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat F(ab')<sub>2</sub>; no trace of albumin, whole molecule IgG or Fc is detected. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strongly acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 1.05 mg/mL; F/P Ratio: 5.2 mol/mol
Conjugate FITC
Formulation Details Purified
Host Goat
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Pack Size 2 mg
Physical Appearance Liquid
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Presentation Liquid
Product Families Description Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')<sub>2</sub> fragments to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Mouse; (IgG; IgA; IgM)
Specificity This product shows reactivity to mouse immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.