Fluorescein-Conjugated Goat IgG Fraction To Mouse IgG (No Cross To Rat)

Fluorescein-Conjugated Goat IgG Fraction To Mouse IgG (No Cross To Rat)

MDL Number: MFCD00162642

Packaging size: 2 mL

SKU 0855503
Price:
$525.90
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse IgG (no cross to rat) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat Anti Mouse IgG is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855503
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to rat IgG or mouse non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat Anti Mouse IgG is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 14.9 mg/mL; Antibody Protein: 3.0 mg/mL
Conjugate FITC
Formulation Lyophilized
Host Goat
Immunogen Mouse IgG
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
MDL # MFCD00162642
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Presentation lyophilized
Product Families Description Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse IgG (no cross to rat) and buffer salts.
Purity Purified
Solubility Soluble in water
Species Reactivity Mouse; (No Cross To Rat)
Specificity This product shows reactivity to mouse IgG. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.