Goat Affinity Purified F(Ab')2 Fragment To Mouse IgG (Whole Molecule)

Goat Affinity Purified F(Ab')2 Fragment To Mouse IgG (Whole Molecule)

MDL Number: MFCD00162638

Packaging size: 2 mg

SKU 0855487
Price:
$204.60
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Description

Product Description

Product is goat affinity purified F(ab')2 fragments to mouse IgG (whole molecule) and buffer salts.

Application Notes

Goat Affinity Purified F(ab')2 Fragment to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855487
Analysis Note The total protein is measured by absorbance (A<sub>280</sub>nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified F(ab')<sub>2</sub> fragment is tested for purity at 10 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat Affinity Purified F(ab')<sub>2</sub> Fragment to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 1.2 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Purified
Host Goat
Immunogen IgG purified from pooled normal mouse serum
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
MDL # MFCD00162638
Pack Size 2 mg
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Liquid
Preparation Method The affinity purified F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Presentation Affinity purified liquid F(ab´)<sub>2</sub>
Product Families Description Product is goat affinity purified F(ab')<sub>2</sub> fragments to mouse IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Recommended Replacement For 0855488 | 0867030
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 1-2 days at 2-8°C unless preservative is added. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.