Goat IgG Fraction To Mouse IgG Fc

Goat IgG Fraction To Mouse IgG Fc

Synonyms Anti-Mouse

MDL Number: MFCD00162665

Packaging size: 2 mL

SKU 0855459
Price:
$199.10
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Description

Product Description

Product is the lyophilized powder of goat IgG fraction to mouse IgG Fc and buffer salts.

Application Notes

Goat IgG Fraction to Mouse IgG Fc is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855459
Alternate Names Anti-Mouse
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to F(ab')<sub>2</sub>, IgA, IgM, or non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat IgG Fraction to Mouse IgG Fc is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Concentration Total Protein: 20.3 mg/mL; Antibody Protein: 4 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Immunogen Mouse IgG
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
MDL # MFCD00162665
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Lyophilized powder
Preparation Method The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Presentation Lyophilized IgG
Product Families Description Product is the lyophilized powder of goat IgG fraction to mouse IgG Fc and buffer salts.
Solubility Soluble in water
Species Reactivity Mouse; IgG Fc
Specificity It shows reactivity to mouse Fc and IgG; cross-reactivity to other species may exist.
Storage and Handling Store lyophilized product at 2-8°C. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.