Rabbit IgG Fraction To Mouse IgG (Whole Molecule)

Rabbit IgG Fraction To Mouse IgG (Whole Molecule)

MDL Number: MFCD00162638

Packaging size: 2 mL

SKU 0855456
Price:
$182.70
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Description

Product Description

Product is the lyophilized powder of rabbit IgG fraction to mouse IgG (whole molecule) and buffer salts.

Application Notes

Rabbit IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)

Specifications
SKU 0855456
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Rabbit IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Certifications The product is mostly rabbit IgG.
Concentration Total Protein: 10.7 mg/mL; Antibody Protein: 4 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rabbit
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)
MDL # MFCD00162638
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Physical Appearance lyophilized powder
Preparation Method The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation Lyophilized IgG
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store product at 2-8°C. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Indirect ELISA: 1:80,000; quantitative precipitin assay: 2.0 mg/mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.