Peroxidase-Conjugated Goat IgG Fraction To Guinea Pig IgG (Whole Molecule)
Product Description
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to guinea pig IgG (whole molecule) and buffer salts.
Application Notes
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
| SKU | 0855391 |
| Analysis Note | The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with guinea pig IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected. |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). |
| Concentration | Total Protein: 15.9 mg/mL Titer: 1:176;000 |
| Conjugate | HRP |
| Formulation Details | Lyophilized |
| Host | Goat |
| Applications | Immunohistochemistry | Immunoblotting |ELISA |
| Pack Size | 2 mL |
| Physical Appearance | Lyophilized powder |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. No preservative has been added. |
| Presentation | lyophilized |
| Purity | Purified |
| Recommended Replacement For | 0855392 | 0861208 |
| Solubility | Soluble in water |
| Species Reactivity | Pig; (Whole Molecule) |
| Specificity | It shows reactivity to guinea pig IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Storage and Handling | Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower. |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |