Fluorescein-Conjugated Goat IgG Fraction To Dog IgG (Whole Molecule)

Fluorescein-Conjugated Goat IgG Fraction To Dog IgG (Whole Molecule)

MDL Number: MFCD00221535

Packaging size: 2 mL

SKU 0855325
Price:
$213.35
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to dog IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG Fraction to Dog IgG (Whole Molecule) is used as a reagent in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855325
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat IgG Fraction to Dog IgG (Whole Molecule) is used as a reagent in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 21.9 mg/ml F/P Ratio: 5.95 mol/mol Antibody Protein: 8 mg/m
Conjugate FITC
Formulation Lyophilized
Host Goat
Immunogen Dog IgG
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
MDL # MFCD00221535
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Physical Appearance lyophilized powder
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Solubility Soluble in water
Species Reactivity Dog; (Whole Molecule)
Specificity This product shows reactivity to dog IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: Direct immunofluorescence: 1:32
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.