Peroxidase-Conjugated Goat IgG Fraction To Human Fibronectin
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human fibronectin and buffer salts.
Peroxidase-Conjugated Goat IgG Fraction to Human Fibronectin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry
|Analysis Note||The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with human fibronectin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis; No trace of albumin is detected. Antibody activity to human fibrinogen or other serum proteins is not present.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Peroxidase-Conjugated Goat IgG Fraction to Human Fibronectin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors).|
|Certifications||The antibody is predominantly goat IgG.|
|Concentration||Total Protein: 63.2 mg/mL; Titer: 1:38;800|
|Applications||Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry|
|Pack Size||2 mL|
|Preparation Method||Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.|
|Presentation||Lyophilized PBS with 0.01% Thiomersal | Lyophilized IgG|
|Recommended Replacement For||855037|
|Research Areas||Cytoskeletal Signaling||Angiogenesis||Neural Stem Cells||Mesenchymal Stem Cells||Metastasis||Developmental Signaling|
|Solubility||Soluble in water|
|Specificity||It shows reactivity to human fibronectin; cross-reactivity to other species may exist. Antibody activity to human fibrinogen or other serum proteins is not present.|
|Storage and Handling||Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.|
|Typical Working Concentration||Dilution Recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA|