Rabbit Antiserum To Human Serum
Rabbit Antiserum to Human Serum is the lyophilized powder of rabbit antiserum to human serum and buffer salts.
Rabbit Antiserum to Human Serum is used in immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)
|Analysis Note||The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized with an in-house control by immunoelectrophoresis. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Rabbit Antiserum to Human Serum is used in immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.|
|Concentration||Total Protein: 34.5 mg/mL|
|Immunogen||Pooled normal human serum|
|Applications||Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)|
|Pack Size||2 mL|
|Physical Appearance||lyophilized powder|
|Preparation Method||The antiserum is prepared by hyperimmunizing rabbits with human serum. The antiserum is delipidated and dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, wtih 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.|
|Recommended Replacement For||865029|
|Solubility||Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Specificity||It shows reactivity to human serum; cross-reactivity to other species may exist.|
|Storage and Handling||Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.|