Purified Mouse Myeloma Protein IgG2A (Kappa) UPC 10

Purified Mouse Myeloma Protein IgG2A (Kappa) UPC 10

Synonyms Mouse IgG2a/k

MDL Number: MFCD00164800

Packaging size: 1 mg

SKU 0850328
Price:
$133.80
In Stock
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Description

Product Description

Product is purified mouse IgG2a (Kappa) and buffer. Mouse IgG2a (Kappa) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Control

Specifications
SKU 0850328
Alternate Names Mouse IgG2a/k
Analysis Note The total protein is determined by absorbance at 280nm. The product contains 5 mg of mouse IgG2a. The product is tested for purity at 1.0 mg/mL using immunoelectrophoresis. The product is mouse IgG2a; no trace of IgG1, IgG2b, IgG3, IgA, IgM or non-immunoglobulin serum proteins is detected.
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Clone Name UPC 10
Concentration Total Protein: 5.0 mg/mL
Conjugate Unconjugated
Formulation Purified
Host Mouse
Isotype IgG
Applications Immunoassays | ELISA | Control
MDL # MFCD00164800
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Puried product in buffer
Preparation Method Mouse IgG2a (j) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.01M Sodium Phosphate, 0.13M sodium chloride, pH 7.2, and vialed.
Presentation Purified proteins are dialyzed into 0.02M Tris; 0.15M sodium chloride; pH 8.1.
Purity Affinity Purified
Recommended Replacement For 850329
Research Areas Immunoassays
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2a. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2a; no trace of IgG1; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Storage and Handling Store unopened product at -70°C. Store opened material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -70°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.