Purified Mouse Myeloma Protein IgA (Lambda2) MOPC 315

Purified Mouse Myeloma Protein IgA (Lambda2) MOPC 315

MDL Number: MFCD00162614

Packaging size: 1 mL

SKU 0850325
Price:
$151.80
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Description

Product Description

Mouse IgA (l2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Ouchterlony Double Diffusion

Specifications
SKU 0850325
Analysis Note The total protein is determined using the Biuret procedure with bovine albumin as the standard and a TRIS blank. The product contains 1 mg of mouse IgA. The product is tested for purity at 1.0 mg/mL using immunoelectrophoresis. The product is mouse IgA; no trace of IgG, IgM or non-immunoglobulin serum proteins is detected.
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.
Clone Name MOPC 315
Concentration 1.1 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgA
Applications Immunoassays | Ouchterlony Double Diffusion
Pack Size 1 mL
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Liquid
Preparation Method Mouse IgA (λ2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Presentation Purified proteins are dialyzed into 0.01M sodium phosphate; 0.13M sodium chloride; pH 7.2; with 0.02% sodium azide.
Product Families Description Product is purified mouse IgA (λ?) and buffer.
Purity Affinity Purified
Solubility Soluble in Water
Specificity The total protein is determined using the Biuret procedure with bovine albumin as the standard and a phosphate blank. The product contains 1 mg of mouse IgA. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgA; no trace of IgG; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Storage and Handling Store unopened product at -70°C. Store opened material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -70°C.
Typical Working Concentration Detection of antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL .
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.