Urea, Ultra-Pure (1lb Size)

Urea, Ultra-Pure (1lb Size)

Synonyms Carbamide; Isourea; Carbonyldiamide; Carbonyldiamine; Ureophil; Carbamimidic acid; Pseudourea; Ureaphil; Urevert

CAS Number: 57-13-6 Molecular Formula: CH4N2O Molecular Weight: 60.06

Beilstein Registry Number: 635724 EC Number: 200-315-5 MDL Number: MFCD00008022

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SKU 04821530-CF
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Description

Product Description

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

Application Notes

Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Denaturation; Protein Electrophoresis; SDS-PAGE

Specifications
SKU 04821530-CF
Alternate Names Carbamide; Isourea; Carbonyldiamide; Carbonyldiamine; Ureophil; Carbamimidic acid; Pseudourea; Ureaphil; Urevert
Application Notes Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Beilstein Registry Number 635724
Biochemical Physiological Actions Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first.Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Boiling Point Decomposes at 135 °C
Density 1.335 g/cm3 at 20 °C
EC Number 200-315-5
Foreign Activity Cyanate; DNase; RNase; Cyanate ;protease none detected
Grade Ultra Pure
Heavy Metals Lead ≤1 ppm, Iron ≤1 ppm and copper ≤1 ppm
Applications Denaturation; Protein Electrophoresis; SDS-PAGE
MDL # MFCD00008022
Melting Point 130 - 140°C
Molecular Formula CH4N2O
Molecular Weight 60.06
Partition Coefficient log Pow: -2,59 - -1,59
pH 8.5 ± 1.0 (7M aq soln)
Physical Appearance White Prills
Preparation Method Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature
Purity ≥99%
RTECS Number YR6250000
Solubility One gram dissolves in 1 mL water, 10 mL 95% ethanol, 1 mL boiling 95% ethanol, 20 mL absolute ethanol, 6 mL methanol, 2 mL glycerol; Soluble in concentrated hydrochloric acid; almost insoluble in chloroform, ether.
Storage and Handling Room Temperature
Typical Working Concentration Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance OD260 (7M aq soln) ≤0.05