Dino

Agarose

Agarose

Synonyms 3,6-Anhydro-a-L-galacto-b-D-galactan;FastLane agarose; Indubiose A4; NuSieve GTG; Odigose;Seakem; Sepharose

CAS Number: 9012-36-6

EC Number: 232-731-8 MDL Number: MFCD00081294

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SKU 04820721-CF
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Description

Product Description

Agarose

Application Notes

Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Electrophoresis;Nucleic Acid Separation;Chromatography;Restriction Mapping; PCR.

Specifications
SKU 04820721-CF
Analysis Note The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.Gel strength - the force that must be applied to a gel to cause it to fracture.Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application Notes Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles.
EC Number 232-731-8
Electroendosmosis 0.11 - 0.15
Foreign Activity DNase; RNase; none detected
Gel Strength ≥600 gm/cm2
Gel Temperature 35 - 37°C
Grade Electrophoresis Grade
Applications Electrophoresis;Nucleic Acid Separation;Chromatography;Restriction Mapping; PCR.
Moisture Content ≤5%
Optical Rotation ≤0.15%
Physical Appearance White Powder
Product Families Description Agarose
Product Overview Agarose
Source Soluble in water.
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.