Guanidine Hydrochloride

Guanidine Hydrochloride

Synonyms Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride

CAS Number: 50-01-1 Molecular Formula: CH5N3·HCl Molecular Weight: 95.53 g/mol

Beilstein Registry Number: 3591990 EC Number: 200-002-3 MDL Number: MFCD00013026

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SKU 04820512-CF
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Description

Product Description

Guanidine Hydrochloride

Application Notes

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Chaotropic agent

Specifications
SKU 04820512-CF
Alternate Names Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride
Application Notes Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.
Beilstein Registry Number 3591990
Density 1.3 g/cm³
EC Number 200-002-3
Format Crystalline powder
Grade Ultra Pure grade
Applications Chaotropic agent
Loss on Drying ≤1.0%
MDL # MFCD00013026
Melting Point 178-185°C
Molecular Formula CH5N3·HCl
Molecular Weight 95.53 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance White Crystalline Powder
pKa 13.6 (Lit.)
Product Families Description Guanidine hydrochloride
Product Overview Guanidine Hydrochloride
Purity ≥99%
RTECS Number MF4300000
Safety Symbol GHS07
Solubility in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, g/100ml at 20°C: 215
Storage and Handling Store at Room Temperature (15-30 °C). Store Desiccated.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance OD260: <0.03 (6.0 M aq soln)