Tris

Tris

Synonyms Tris-[hydroxymethyl]aminomethane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; Tromethamine; Trometamol

CAS Number: 77-86-1 Molecular Formula: NH2C(CH2OH)3 Molecular Weight: 121.136 g/mol

Beilstein Registry Number: 741883 EC Number: 201-064-4

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SKU 04819620-CF
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Description

Product Description

Tris

Application Notes

Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Electrophoresis

Specifications
SKU 04819620-CF
Alternate Names Tris-[hydroxymethyl]aminomethane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; Tromethamine; Trometamol
Application Notes Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.
Beilstein Registry Number 741883
Boiling Point 219-220 deg C at 10 mm Hg
EC Number 201-064-4
Format powder
Grade Ultra Pure
Hazard Statements H315-H319-H335
Heavy Metals (as Pb)
Insoluble Matter ≤5 ppmIron (Fe)
Isoelectric Point ≤5 ppm
Applications Electrophoresis
Mean Pore Size ≤1%
Melting Point 171-172 deg C
Moisture Content MFCD00004679
Molecular Formula NH2C(CH2OH)3
Molecular Weight 121.136 g/mol
Optical Rotation 02103133.1||02152176.1||04819619||04819638||04821557||02103132.5||04819623||0219455780
pH pH of 0.1 molar aq soln = 10.4
Physical Appearance White crystalline powder
pKa Dust mask type , Eyeshields, Gloves
Potency (5% aq soln)
Preparation Method pKa at 25°C= 8.1
Product Families Description Tris
Product Overview Tris
Purity ≥99.9%
Refractive Index T
Residue on Ignition ≤0.1
RTECS Number TY2900000
Solubility 79.1 mg/mL in <a class="pubchem-internal-link CID-174" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethylene%20glycol">ethylene glycol</a>; 26 mg/mL in <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>; 14.6 mg/mL in anhyd <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 22 mg/mL in 95% <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 14 mg/mL in <a class="pubchem-internal-link CID-6228" href="https://pubchem.ncbi.nlm.nih.gov/compound/dimethyl-formamide">dimethyl formamide</a>; 20 mg/mL in <a class="pubchem-internal-link CID-180" href="https://pubchem.ncbi.nlm.nih.gov/compound/acetone">acetone</a>; 0.5 mg/mL in <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a>; 0.4 mg/mL in olive oil; 0.1 mg/mL in <a class="pubchem-internal-link CID-8078" href="https://pubchem.ncbi.nlm.nih.gov/compound/cyclohexane">cyclohexane</a>; 0.05 mg/mL in <a class="pubchem-internal-link CID-6212" href="https://pubchem.ncbi.nlm.nih.gov/compound/chloroform">chloroform</a>; less than 0.05 mg/mL in <a class="pubchem-internal-link CID-5943" href="https://pubchem.ncbi.nlm.nih.gov/compound/carbon%20tetrachloride">carbon tetrachloride</a>
Sterility Soluble in water (550 mg/mL); ethylene glycol (79.1 mg/mL); methanol (26 mg/mL); anhydrous ethanol (14.6 mg/mL); 95% ethanol (22.0 mg/mL); DMF (14 mg/mL); acetone (2 mg/mL); ethyl acetate (0.5 mg/mL); olive oil (0.4 mg/mL); and chloroform (0.05 mg/mL)
Storage and Handling Room Temperature; desiccate
Surface Area Tris solutions can be autoclaved (121°C, 15 psi, 15 minutes) or sterile filtered.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Pressure 2.2X10-5 mm Hg at 25 deg C (est)